Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection

/ / Faculty Research in Latin America, Research

UC BERKELEY AUTHOR: Eva Harris

DATE OF PUBLICATION: May 2016

REGION: Managua, Nicaragua

REFERENCE: Waggoner JJ, Ballesteros G, Gresh L, et al. Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection. J Clin Virol. 2016;78:57-61.

SUMMARY/ABSTRACT: Dengue virus (DENV) and chikungunya virus (CHIKV) now co-circulate throughout tropical regions of the world, with billions of people living at risk of infection. The differentiation of these infections is important for epidemiologic surveillance as well as clinical care, though widely-used molecular diagnostics for DENV and CHIKV require the performance of two to four separate PCR reactions for detection.

ACCESS: Link to Pubmed